This study reevaluated porcine MuSC sorting strategies, introducing a novel marker combination—CD31, CD45, JAM1, ITGA5, and ITGA7—to classify muscle mononuclear cells into three groups. Immunofluorescence and RNA-sequencing confirmed these as MuSCs, smooth muscle cells (SMCs), and fibro-adipogenic progenitors (FAPs), each expressing characteristic markers. The refined sorting method yielded higher-purity MuSCs with superior myogenic properties after passaging. This approach enables simultaneous isolation of MuSCs, SMCs, and FAPs from porcine tissue, offering a high-quality seed cell source for cultured meat.
Source: Isolation and purification of different high-purity cell populations from pig muscle tissue